ELISA/assay

Inter Alpha-Globulin Inhibitor H3 (ITIH3) ELISA Kit

MBS2023717

96 Strip Wells

780 USD

ELISA Kit

Inter Alpha-Globulin Inhibitor H3 (ITIH3) ELISA Kit

Inter Alpha-Globulin Inhibitor H3 (ITIH3)

H3P; SHAP; Pre-Alpha(Globulin)Inhibitor,H3 Polypeptide; Serum-derived hyaluronan-associated protein

inter-alpha-trypsin inhibitor heavy chain H3 preproprotein; Inter-alpha-trypsin inhibitor heavy chain H3; inter-alpha-trypsin inhibitor heavy chain H3; inter-alpha-trypsin inhibitor heavy chain 3; Serum-derived hyaluronan-associated protein; SHAP

ITIH3

ITIH3; ITIH3; H3P; SHAP; ITI-HC3; ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; SHAP

ITIH3_HUMAN

Human

890

This assay has high sensitivity and excellent specificity for detection of Inter Alpha-Globulin Inhibitor H3 (ITIH3). No significant cross-reactivity or interference between Inter Alpha-Globulin Inhibitor H3 (ITIH3) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 6.25-400ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 2.52ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inter Alpha-Globulin Inhibitor H3 (ITIH3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inter Alpha-Globulin Inhibitor H3 (ITIH3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H3 (ITIH3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H3 (ITIH3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H3 (ITIH3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Inter Alpha-Globulin Inhibitor H3 (ITIH3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

133925809

NP_002208.3

NM_002217.3

99,328 Da

This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. Polymorphisms of this gene may be associated with increased risk for schizophrenia and major depressive disorder. This gene is present in an inter-alpha-trypsin inhibitor family gene cluster on chromosome 3. [provided by RefSeq, Jul 2015]

96 Strip Wells

780 USD