Matrilin 2 (MATN2) ELISA Kit | MyBioSource MBS2023688 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Matrilin 2 (MATN2) ELISA Kit

catalog :

MBS2023688

quantity :

48-Strip-Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2023688

products type :

ELISA Kit

products full name :

Matrilin 2 (MATN2) ELISA Kit

products short name :

Matrilin 2 (MATN2)

other names :

matrilin-2 isoform b; Matrilin-2; matrilin-2; matrilin 2

products gene name :

MATN2

other gene names :

MATN2; MATN2

uniprot entry name :

MATN2_HUMAN

reactivity :

Mouse

sequence length :

672

specificity :

This assay has high sensitivity and excellent specificity for detection of MATN2. No significant cross-reactivity or interference between MATN2 and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Mouse tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: 0.056ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MATN2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MATN2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MATN2 in mouse tissue homogenates, cell lysates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to MATN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MATN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MATN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MATN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

62548862

ncbi acc num :

NP_085072.2

ncbi gb acc num :

NM_030583.2

uniprot acc num :

O00339

ncbi mol weight :

75,251 Da

ncbi summary :

This gene encodes a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains five von Willebrand factor A domains. The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

MATN2: Involved in matrix assembly. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Extracellular matrix; Secreted. Chromosomal Location of Human Ortholog: 8q22. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; basement membrane. Molecular Function: protein binding; calcium ion binding. Biological Process: dendrite regeneration; axon guidance; neuron migration; response to axon injury; glial cell migration

size1 :

48-Strip-Wells

price1 :

575 USD

size2 :

96-Strip-Wells

price2 :

785

size3 :

5x96-Strip-Wells

price3 :

3055

size4 :

10x96-Strip-Wells

price4 :

5495

more info or order :

MyBioSource product webpage