ELISA/assay

Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) ELISA Kit

MBS2023668

48-Strip-Wells

610 USD

ELISA Kit

Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) ELISA Kit

Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1)

IDGFL; ADGF; ADA2; Cat Eye Syndrome Critical Region Protein 1

Cat eye syndrome chromosome region, candidate 1; Adenosine deaminase CECR1; adenosine deaminase CECR1; cat eye syndrome chromosome region, candidate 1; Cat eye syndrome critical region protein 1

CECR1

CECR1; CECR1; PAN; ADA2; ADGF; IDGFL; SNEDS; ADA2; ADGF; IDGFL

CECR1_HUMAN

Human

270

This assay has high sensitivity and excellent specificity for detection of Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). No significant cross-reactivity or interference between Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.119ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

30704990

AAH51755.1

Q9NZK5

30,668 Da

This gene encodes a member of a subfamily of the adenosine deaminase protein family. The encoded protein is one of two adenosine deaminases found in humans, which regulate levels of the signaling molecule, adenosine. The encoded protein is secreted from monocytes undergoing differentiation and may regulate cell proliferation and differentiation. This gene may be responsible for some of the phenotypic features associated with cat eye syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

CECR1: Adenosine deaminase that may contribute to the degradation of extracellular adenosine, a signaling molecule that controls a variety of cellular responses. Requires elevated adenosine levels for optimal enzyme activity. Binds to cell surfaces via proteoglycans and may play a role in the regulation of cell proliferation and differentiation, independently of its enzyme activity. Belongs to the adenosine and AMP deaminases family. ADGF subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; EC 3.5.4.4; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 22q11.2. Cellular Component: extracellular space; cytosol. Molecular Function: adenosine deaminase activity; heparin binding; proteoglycan binding; protein homodimerization activity; growth factor activity; zinc ion binding; adenosine receptor binding. Biological Process: adenosine catabolic process; multicellular organismal development; hypoxanthine salvage; inosine biosynthetic process. Disease: Polyarteritis Nodosa, Childhood-onset; Sneddon Syndrome

48-Strip-Wells

610 USD

96-Strip-Wells

830

5x96-Strip-Wells

3265

10x96-Strip-Wells

5870