ELISA/assay

Corticotropin Releasing Hormone (CRH) ELISA Kit

MBS2023613

24-Strip-Wells

255 USD

ELISA Kit

Corticotropin Releasing Hormone (CRH) ELISA Kit

[Corticotropin Releasing Hormone (CRH)]

[CRF; Corticotropin Releasing Factor; Corticoliberin]

[corticotropin releasing hormone; Corticoliberin; corticoliberin; corticotropin releasing hormone; Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone]

[CRH]

[CRH; CRH; CRF; CRH1; CRF]

CRF_HUMAN

Human

196

This assay has high sensitivity and excellent specificity for detection of cotinine. No significant cross-reactivity or interference between cotinine and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 617.3-50,000pg/mL. Sensitivity: 284.2pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level cotinine were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level cotinine were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Endocrinology; Cardiovascular biology; Neuroscience; Hormone metabolism

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of cotinine in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to cotinine has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled cotinine and unlabeled cotinine (Standards or samples) with the pre-coated antibody specific to cotinine. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of cotinine in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of cotinine in the sample.

119607303

EAW86897.1

P06850

21,422 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Class B/2 (Secretin Family Receptors) Pathway (106378); Corticotropin-releasing Hormone Pathway (920957); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Long-term Depression Pathway (83086)

Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]

CRH: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8q13. Cellular Component: extracellular space; cytoplasm; extracellular region; perikaryon. Molecular Function: corticotropin-releasing hormone receptor 1 binding; neuropeptide hormone activity; protein binding; hormone activity; corticotropin-releasing hormone receptor 2 binding; receptor binding; adrenocorticotropin-releasing hormone activity. Biological Process: synaptic transmission, dopaminergic; diterpenoid metabolic process; negative regulation of circadian sleep/wake cycle, REM sleep; hypothalamus development; positive regulation of cortisol secretion; induction of apoptosis by hormones; response to pain; female pregnancy; signal transduction; positive regulation of adrenocorticotropic hormone secretion; synaptic transmission; negative regulation of luteinizing hormone secretion; learning and/or memory; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; feeding behavior; negative regulation of blood pressure; parturition; response to electrical stimulus; inflammatory response; associative learning; response to corticosterone stimulus; response to drug; adrenal gland development; response to ether; response to ethanol; long-term memory; ion homeostasis; response to estrogen stimulus; regulation of serotonin secretion; negative regulation of epinephrine secretion; positive regulation of protein amino acid phosphorylation; glucocorticoid biosynthetic process; lung development. Disease: Acth Deficiency, Isolated

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445