Neuromedin B (NMB) ELISA Kit | MyBioSource MBS2023611 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Neuromedin B (NMB) ELISA Kit

catalog :

MBS2023611

quantity :

48-Strip-Wells

price :

515 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2023611

products type :

ELISA Kit

products full name :

Neuromedin B (NMB) ELISA Kit

products short name :

Neuromedin B (NMB)

products name syn :

NM-B

other names :

neuromedin-B isoform 2 preproprotein; Neuromedin-B; neuromedin-B; neuromedin B

products gene name :

NMB

other gene names :

NMB; NMB

uniprot entry name :

NMB_HUMAN

reactivity :

Human

sequence length :

154

specificity :

This assay has high sensitivity and excellent specificity for detection of Neuromedin B (NMB). No significant cross-reactivity or interference between Neuromedin B (NMB) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 123.46-10000pg/mL. Sensitivity: Typically less than 43.5pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuromedin B (NMB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuromedin B (NMB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37 degree C;. 3. Aspirate and wash 3 times;. 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 5. Aspirate and wash 5 times;. 6. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 7. Add 50uL Stop Solution. Read at 450 nm immediately.

products description :

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuromedin B (NMB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuromedin B (NMB) and unlabeled Neuromedin B (NMB) (Standards or samples) with the pre-coated antibody specific to Neuromedin B (NMB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuromedin B (NMB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuromedin B (NMB) in the sample.

ncbi gi num :

45505145

ncbi acc num :

NP_995580.1

ncbi gb acc num :

NM_205858.1

uniprot acc num :

P08949

ncbi mol weight :

16,309 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114)

ncbi summary :

This gene encodes a member of the bombesin-like family of neuropeptides, which negatively regulate eating behavior. The encoded protein may regulate colonic smooth muscle contraction through binding to its cognate receptor, the neuromedin B receptor (NMBR). Polymorphisms of this gene may be associated with hunger, weight gain and obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

uniprot summary :

NMB: Stimulates smooth muscle contraction in a manner similar to that of bombesin. Belongs to the bombesin/neuromedin-B/ranatensin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 15q22-qter. Cellular Component: neuron projection; extracellular region. Molecular Function: hormone activity; neuromedin B receptor binding. Biological Process: elevation of cytosolic calcium ion concentration; cell-cell signaling; neuropeptide signaling pathway; negative regulation of hormone secretion; positive regulation of cell proliferation; positive regulation of hormone secretion; glucose homeostasis; arachidonic acid secretion; signal transduction

size1 :

48-Strip-Wells

price1 :

515 USD

size2 :

96-Strip-Wells

price2 :

695

size3 :

5x96-Strip-Wells

price3 :

2685

size4 :

10x96-Strip-Wells

price4 :

4850

more info or order :

MyBioSource product webpage