ELISA/assay

High Sensitive Interleukin 6 (IL6) ELISA Kit

MBS2023586

24-Strip-Wells

290 USD

ELISA Kit

High Sensitive Interleukin 6 (IL6) ELISA Kit

[Interleukin 6 (IL6)]

[MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor]

[interleukin-6; Interleukin-6; interleukin-6; interleukin 6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2]

[IL6]

[IL6; IL6; HGF; HSF; BSF2; IL-6; IFNB2; IFNB2; IL-6; BSF-2; CDF; IFN-beta-2]

IL6_HUMAN

Bovine

212

This assay has high sensitivity and excellent specificity for detection of High Sensitive Interleukin 6 (IL6). No significant cross-reactivity or interference between High Sensitive Interleukin 6 (IL6) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 1.56-100pg/mL. Sensitivity: < 0.45pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 6 (IL6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Cytokine; Tumor immunity; Infection immunity; Cardiovascular biology

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of High Sensitive Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

10834984

NP_000591.1

NM_000600.3

P05231

23,718 Da

ARMS-mediated Activation Pathway (106466); ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (106400); Adipogenesis Pathway (198832); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Axon Guidance Pathway (105688); Cellular Senescence Pathway (905991)

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]

IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7p21. Cellular Component: extracellular space; cytoplasm; extracellular region; interleukin-6 receptor complex; external side of plasma membrane. Molecular Function: protein binding; interleukin-6 receptor binding; growth factor activity; cytokine activity. Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; response to glucocorticoid stimulus; negative regulation of cytokine secretion; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; bone remodeling; glucose homeostasis; positive regulation of tyrosine phosphorylation of Stat3 protein; response to caffeine; activation of NF-kappaB transcription factor; response to antibiotic; muscle maintenance; monocyte chemotaxis; regulation of cell shape; positive regulation of T-helper 2 cell differentiation; positive regulation of acute inflammatory response; negative regulation of gluconeogenesis; acute-phase response; positive regulation of T cell proliferation; response to electrical stimulus; cell growth; defense response to virus; neurite development; response to drug; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of interleukin-6 production; response to amino acid stimulus; defense response to protozoan; negative regulation of fat cell differentiation; positive regulation of chemokine production; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; response to heat; cell redox homeostasis; positive regulation of B cell activation; negative regulation of protein kinase activity; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; response to calcium ion; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of muscle development; positive regulation of translation; positive regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; neutrophil mediated immunity; negative regulation of caspase activity; regulation of circadian sleep/wake cycle, non-REM sleep; defense response to Gram-negative bacterium; response to insulin stimulus; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to peptidoglycan; positive regulation of cell proliferation; hepatic immune response; inflammatory response; aging; negative regulation of chemokine biosynthetic process; response to nutrient levels; cytokine and chemokine mediated signaling pathway; positive regulation of immunoglobulin secretion; regulation of angiogenesis; endocrine pancreas development; positive regulation of peptidyl-serine phosphorylation; humoral immune response; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; response to cold; positive regulation of neuron differentiation; positive regulation of DNA replication; positive regulation of transmission of nerve impulse. Disease: Rheumatoid Arthritis, Systemic Juvenile; Arteriovenous Malformations Of The Brain; Kaposi Sarcoma, Susceptibility To; Inflammatory Bowel Disease 1

24-Strip-Wells

290 USD

48-Strip-Wells

550

96-Strip-Wells

745

5x96-Strip-Wells

3035

10x96-Strip-Wells

5475