Lysyl Oxidase (LOX) ELISA Kit | MyBioSource MBS2023535 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Lysyl Oxidase (LOX) ELISA Kit

catalog :

MBS2023535

quantity :

24-Strip-Wells

price :

270 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2023535

products type :

ELISA Kit

products full name :

Lysyl Oxidase (LOX) ELISA Kit

products short name :

[Lysyl Oxidase (LOX)]

products name syn :

[Protein-Lysine 6-Oxidase]

other names :

[lysyl oxidase; Protein-lysine 6-oxidase; protein-lysine 6-oxidase; lysyl oxidase; Lysyl oxidase]

products gene name :

[LOX]

other gene names :

[LOX; LOX]

uniprot entry name :

LYOX_HUMAN

reactivity :

Human

sequence length :

417

specificity :

This assay has high sensitivity and excellent specificity for detection of Lysyl Oxidase (LOX). No significant cross-reactivity or interference between Lysyl Oxidase (LOX) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 0.156-10ng/mL. Sensitivity: < 0.056ng/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Enzyme & Kinase

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase (LOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl Oxidase (LOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl Oxidase (LOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Lysyl Oxidase (LOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

257065

ncbi acc num :

AAB23549.1

uniprot acc num :

P28300

ncbi mol weight :

46,944 Da

ncbi pathways :

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

uniprot summary :

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family. Protein type: Oxidoreductase; Secreted; EC 1.4.3.13; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q23.2. Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; nucleus. Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity. Biological Process: response to drug; elastic fiber assembly; blood vessel development; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; response to steroid hormone stimulus; protein modification process; lung development. Disease: Cutis Laxa, Autosomal Recessive, Type Ia

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

665

size4 :

5x96-Strip-Wells

price4 :

2715

size5 :

10x96-Strip-Wells

price5 :

4895

more info or order :

MyBioSource product webpage