WNT Inhibitory Factor 1 (WIF1) ELISA Kit | MyBioSource MBS2023495 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

WNT Inhibitory Factor 1 (WIF1) ELISA Kit

catalog :

MBS2023495

quantity :

48-Strip-Wells

price :

610 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2023495

products type :

ELISA Kit

products full name :

WNT Inhibitory Factor 1 (WIF1) ELISA Kit

products short name :

WNT Inhibitory Factor 1 (WIF1)

other names :

wnt inhibitory factor 1; Wnt inhibitory factor 1; wnt inhibitory factor 1; Wnt inhibitory factor 1

products gene name :

WIF1

other gene names :

Wif1; Wif1; WIF-1; AW107799; WIF-1

uniprot entry name :

WIF1_MOUSE

reactivity :

Human

sequence length :

379

specificity :

This assay has high sensitivity and excellent specificity for detection of WNT Inhibitory Factor 1 (WIF1). No significant cross-reactivity or interference between WNT Inhibitory Factor 1 (WIF1) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 5.6pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT Inhibitory Factor 1 (WIF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT Inhibitory Factor 1 (WIF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT Inhibitory Factor 1 (WIF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT Inhibitory Factor 1 (WIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT Inhibitory Factor 1 (WIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of WNT Inhibitory Factor 1 (WIF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6755999

ncbi acc num :

NP_036045.1

ncbi gb acc num :

NM_011915.2

uniprot acc num :

Q9WUA1

ncbi mol weight :

41,590 Da

ncbi pathways :

Disease Pathway (1111319); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1111454); Signal Transduction Pathway (1110342); Signaling By WNT In Cancer Pathway (1111436); Signaling By Wnt Pathway (1110549); TCF Dependent Signaling In Response To WNT Pathway (1110554); Wnt Signaling Pathway (83258); Wnt Signaling Pathway (471); XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway (1111455)

uniprot summary :

WIF1: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. Protein type: Secreted; Inhibitor; Secreted, signal peptide. Cellular Component: extracellular region. Molecular Function: Wnt-protein binding. Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; multicellular organismal development; positive regulation of fat cell differentiation

size1 :

48-Strip-Wells

price1 :

610 USD

size2 :

96-Strip-Wells

price2 :

830

size3 :

5x96-Strip-Wells

price3 :

3265

size4 :

10x96-Strip-Wells

price4 :

5870

more info or order :

MyBioSource product webpage