ELISA/assay

Syndecan 1 (SDC1) ELISA Kit

MBS2023451

24-Strip-Wells

270 USD

ELISA Kit

Syndecan 1 (SDC1) ELISA Kit

[Syndecan 1 (SDC1)]

[CD138; SDC; SYND1; Syndecan Proteoglycan 1]

[syndecan-1; Syndecan-1; syndecan-1; syndecan 1; CD_antigen: CD138]

[SDC1]

[SDC1; SDC1; SDC; CD138; SYND1; syndecan; SDC; SYND1]

SDC1_HUMAN

Rat

310

This assay has high sensitivity and excellent specificity for detection of SDC1. No significant cross-reactivity or interference between SDC1 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Porcine Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.057ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SDC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SDC1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Tumor immunity; Infection immunity; Immunodeficiency

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SDC1 in porcine serum, plasma, tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SDC1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SDC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SDC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SDC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

55749480

NP_001006947.1

NM_001006946.1

P18827

32,462 Da

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Arf6 Trafficking Events Pathway (137954); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Chylomicron-mediated Lipid Transport Pathway (106157); Defective B3GAT3 Causes JDSSDHD Pathway (1127588); Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway (1127592); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1127587); Defective CHST14 Causes EDS, Musculocontractural Type Pathway (1127591)

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family. Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p24.1. Cellular Component: lysosomal lumen; focal adhesion; protein complex; cell surface; integral to plasma membrane; Golgi lumen; cytoplasm; plasma membrane; external side of plasma membrane. Molecular Function: protein C-terminus binding; protein binding; glycoprotein binding. Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; myoblast development; response to cAMP; wound healing; glycosaminoglycan metabolic process; striated muscle cell development; response to toxin; response to glucocorticoid stimulus; pathogenesis; lipoprotein metabolic process; Wnt receptor signaling pathway through beta-catenin; Sertoli cell development; chondroitin sulfate metabolic process; odontogenesis; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; response to hydrogen peroxide; ureteric bud development; carbohydrate metabolic process; response to calcium ion; inflammatory response; retinoid metabolic process

24-Strip-Wells

270 USD

48-Strip-Wells

505

96-Strip-Wells

685

5x96-Strip-Wells

2775

10x96-Strip-Wells

5035