ELISA/assay

Chordin Like Protein 1 (CHRDL1) ELISA Kit

MBS2023449

48-Strip-Wells

565 USD

ELISA Kit

Chordin Like Protein 1 (CHRDL1) ELISA Kit

Chordin Like Protein 1 (CHRDL1)

NRLN1; CHL; VOPT; Ventroptin; Neuralin-1; Neurogenesin-1

chordin-like protein 1 isoform 1; Chordin-like protein 1; chordin-like protein 1; chordin-like 1; Neuralin-1; Neurogenesin-1; Ventroptin

CHRDL1

CHRDL1; CHRDL1; CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1; NRLN1

CRDL1_HUMAN

Human

456

This assay has high sensitivity and excellent specificity for detection of Chordin Like Protein 1 (CHRDL1). No significant cross-reactivity or interference between Chordin Like Protein 1 (CHRDL1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.058ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chordin Like Protein 1 (CHRDL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chordin Like Protein 1 (CHRDL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chordin Like Protein 1 (CHRDL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chordin Like Protein 1 (CHRDL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chordin Like Protein 1 (CHRDL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Chordin Like Protein 1 (CHRDL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

221316561

NP_001137453.1

NM_001143981.1

Q9BU40

52,026 Da

BMP Receptor Signaling Pathway (137948); Signal Transduction Pathway (477114); Signaling By BMP Pathway (106336)

This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

CHRDL1: Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development. Defects in CHRDL1 are the cause of megalocornea type 1, X-linked (MGC1). MGC1 is an eye disorder in which the corneal diameter is bilaterally enlarged (greater than 13 mm) without an increase in intraocular pressure. It may also be referred to as anterior megalophthalmos, since the entire anterior segment is larger than normal. Features of megalocornea in addition to a deep anterior chamber include astigmatic refractive errors, atrophy of the iris stroma, miosis secondary to decreased function of the dilator muscle, iridodonesis, and tremulousness, subluxation, or dislocation of the lens. Whereas most affected individuals exhibit normal ocular function, complications include cataract development and glaucoma following lenticular dislocation or subluxation. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: Xq23. Cellular Component: extracellular region. Biological Process: BMP signaling pathway; neuron differentiation; ossification; eye development; compound eye development; negative regulation of BMP signaling pathway. Disease: Megalocornea

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340