ELISA/assay

Sirtuin 1 (SIRT1) ELISA Kit

MBS2023445

48-Strip-Wells

575 USD

ELISA Kit

Sirtuin 1 (SIRT1) ELISA Kit

Sirtuin 1 (SIRT1)

SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

sirtuin 1, partial; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; sirtuin 1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2

SIRT1

SIRT1; SIRT1; SIR2; hSIR2; SIR2L1; SIR2L1; hSIRT1; hSIR2; 75SirT1

SIR1_HUMAN

Mouse

561

This assay has high sensitivity and excellent specificity for detection of Sirtuin 1 (SIRT1). No significant cross-reactivity or interference between Sirtuin 1 (SIRT1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.067ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sirtuin 1 (SIRT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sirtuin 1 (SIRT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sirtuin 1 (SIRT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sirtuin 1 (SIRT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sirtuin 1 (SIRT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Sirtuin 1 (SIRT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

385880783

AFI98394.1

Q96EB6

61,066 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Androgen Receptor Signaling Pathway (198806); Cellular Response To Heat Stress Pathway (1127553); Cellular Responses To Stress Pathway (645258); E2F Transcription Factor Network Pathway (137934); Energy Metabolism Pathway (198907); Epigenetic Regulation Of Gene Expression Pathway (1127671)

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5 -AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; Nuclear receptor co-regulator; EC 3.5.1.-; Deacetylase. Chromosomal Location of Human Ortholog: 10q21.3. Cellular Component: nucleoplasm; chromatin silencing complex; PML body; mitochondrion; ESC/E(Z) complex; cytoplasm; nuclear chromatin; nucleolus; nuclear inner membrane; nuclear envelope; nuclear heterochromatin; nucleus. Molecular Function: protein C-terminus binding; deacetylase activity; identical protein binding; histone binding; p53 binding; metal ion binding; protein deacetylase activity; transcription factor binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity; bHLH transcription factor binding; mitogen-activated protein kinase binding; histone deacetylase activity; transcription corepressor activity; HLH domain binding; NAD+ ADP-ribosyltransferase activity. Biological Process: establishment and/or maintenance of chromatin architecture; muscle development; viral reproduction; positive regulation of apoptosis; regulation of mitotic cell cycle; protein ubiquitination; positive regulation of caspase activity; negative regulation of prostaglandin biosynthetic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of gene expression, epigenetic; behavioral response to starvation; positive regulation of adaptive immune response; positive regulation of histone H3-K9 methylation; positive regulation of DNA repair; transcription, DNA-dependent; negative regulation of transcription factor activity; pyrimidine dimer repair via nucleotide-excision repair; cell aging; negative regulation of I-kappaB kinase/NF-kappaB cascade; cellular response to starvation; negative regulation of fat cell differentiation; ovulation from ovarian follicle; regulation of endodeoxyribonuclease activity; protein amino acid ADP-ribosylation; cholesterol homeostasis; methylation-dependent chromatin silencing; inhibition of NF-kappaB transcription factor; maintenance of chromatin silencing; negative regulation of phosphorylation; regulation of protein import into nucleus, translocation; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; negative regulation of transcription, DNA-dependent; triacylglycerol mobilization; rRNA processing; peptidyl-lysine acetylation; negative regulation of apoptosis; chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; establishment of chromatin silencing; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; response to insulin stimulus; DNA synthesis during DNA repair; protein amino acid deacetylation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; circadian regulation of gene expression; angiogenesis; DNA replication; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; single strand break repair; positive regulation of chromatin silencing; negative regulation of TOR signaling pathway; negative regulation of protein kinase B signaling cascade; protein destabilization; histone deacetylation; DNA repair; regulation of cell proliferation; negative regulation of helicase activity; response to hydrogen peroxide; fatty acid homeostasis; white fat cell differentiation; cell glucose homeostasis; spermatogenesis; gene expression; negative regulation of cell growth; positive regulation of insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; response to DNA damage stimulus; positive regulation of macroautophagy

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3055

10x96-Strip-Wells

5495