Fibroblast Growth Factor 21 (FGF21) ELISA Kit | MyBioSource MBS2023437 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fibroblast Growth Factor 21 (FGF21) ELISA Kit

catalog :

MBS2023437

quantity :

24-Strip-Wells

price :

225 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2023437

products type :

ELISA Kit

products full name :

Fibroblast Growth Factor 21 (FGF21) ELISA Kit

products short name :

[Fibroblast Growth Factor 21 (FGF21)]

other names :

[fibroblast growth factor 21; Fibroblast growth factor 21; fibroblast growth factor 21; fibroblast growth factor 21]

products gene name :

[FGF21]

other gene names :

[FGF21; FGF21; FGF-21]

uniprot entry name :

FGF21_HUMAN

reactivity :

Human

sequence length :

209

specificity :

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 21 (FGF21). No significant cross-reactivity or interference between Fibroblast Growth Factor 21 (FGF21) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 7.8-500pg/mL. Sensitivity: < 2.7pg/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Cytokine

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 21 (FGF21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 21 (FGF21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 21 (FGF21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Fibroblast Growth Factor 21 (FGF21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

9506597

ncbi acc num :

NP_061986.1

ncbi gb acc num :

NM_019113.2

uniprot acc num :

Q9NSA1

ncbi mol weight :

22,300 Da

ncbi pathways :

MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Melanoma Pathway (83114); Melanoma Pathway (526); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 19q13.33. Cellular Component: extracellular region. Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding. Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction

size1 :

24-Strip-Wells

price1 :

225 USD

size2 :

48-Strip-Wells

price2 :

380

size3 :

96-Strip-Wells

price3 :

500

size4 :

5x96-Strip-Wells

price4 :

2005

size5 :

10x96-Strip-Wells

price5 :

3615

more info or order :

MyBioSource product webpage