ELISA/assay

N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit

MBS2023403

24-Strip-Wells

260 USD

ELISA Kit

N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit

[N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)]

[NT-Pro-BNP; ; N-BNP]

[NT-ProBNP]

Mouse

This assay has high sensitivity and excellent specificity for detection of NT-ProBNP. No significant cross-reactivity or interference between NT-ProBNP and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 30.9-2,500pg/mL. Sensitivity: 11.7pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NT-ProBNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NT-ProBNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Endocrinology; Cardiovascular biology

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NT-ProBNP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to NT-ProBNP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NT-ProBNP and unlabeled NT-ProBNP (Standards or samples) with the pre-coated antibody specific to NT-ProBNP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NT-ProBNP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NT-ProBNP in the sample.

24-Strip-Wells

260 USD

48-Strip-Wells

465

96-Strip-Wells

625

5x96-Strip-Wells

2525

10x96-Strip-Wells

4550