Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit | MyBioSource MBS2023396 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit

catalog :

MBS2023396

quantity :

24-Strip-Wells

price :

270 USD

product information

catalog number :

MBS2023396

products type :

ELISA Kit

products full name :

Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit

products short name :

[Secreted Frizzled Related Protein 1 (SFRP1)]

products name syn :

[FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2]

other names :

[secreted frizzled-related protein 1; Secreted frizzled-related protein 1; secreted frizzled-related protein 1; secreted frizzled-related protein 1]

products gene name :

[SFRP1]

other gene names :

[Sfrp1; Sfrp1; sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik; sFRP-1]

uniprot entry name :

SFRP1_MOUSE

reactivity :

Human

sequence length :

314

specificity :

This assay has high sensitivity and excellent specificity for detection of SERPINB10. No significant cross-reactivity or interference between SERPINB10 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Tissue Homogenates, Cell Lysates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 125-8,000pg/mL. Sensitivity: 54pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SERPINB10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SERPINB10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Signal transduction

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SERPINB10 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SERPINB10. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SERPINB10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SERPINB10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SERPINB10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

65301472

ncbi acc num :

NP_038862.2

ncbi gb acc num :

NM_013834.3

uniprot acc num :

Q8C4U3

ncbi mol weight :

35,413 Da

ncbi pathways :

Disease Pathway (1111319); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1111454); Signal Transduction Pathway (1110342); Signaling By WNT In Cancer Pathway (1111436); Signaling By Wnt Pathway (1110549); TCF Dependent Signaling In Response To WNT Pathway (1110554); Wnt Signaling Pathway NetPath (198351); Wnt Signaling Pathway (83258); Wnt Signaling Pathway (471); XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway (1111455)

uniprot summary :

SFRP1: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Down-regulated in colorectal and breast tumors. Up- regulated in uterine leiomyomas under high estrogenic conditions. Expression, in leiomyomal cells, also increased both under hypoxic and serum deprivation conditions. Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Belongs to the secreted frizzled-related protein (sFRP) family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; plasma membrane; extracellular region; intracellular; cytosol. Molecular Function: heparin binding; identical protein binding; Wnt-protein binding; protein binding; frizzled binding; cysteine-type endopeptidase activity; drug binding. Biological Process: neural tube development; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; somatic stem cell maintenance; multicellular organismal development; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; proteolysis; response to organic cyclic substance; negative regulation of insulin secretion; negative regulation of BMP signaling pathway; anterior/posterior pattern formation; negative regulation of ossification; positive regulation of smoothened signaling pathway; negative regulation of osteoblast differentiation; female gonad development; response to drug; negative regulation of peptidyl-tyrosine phosphorylation; somitogenesis; digestive tract morphogenesis; positive regulation of cell growth; negative regulation of fibroblast proliferation; negative regulation of bone remodeling; positive regulation of fat cell differentiation; hemopoietic progenitor cell differentiation; negative regulation of osteoblast proliferation; neural crest cell fate commitment; regulation of ossification; negative regulation of transcription, DNA-dependent; actin cytoskeleton organization and biogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of osteoclast differentiation; dorsal/ventral axis specification; negative regulation of cell proliferation; positive regulation of cell proliferation; hemopoiesis; cell differentiation; positive regulation of Wnt receptor signaling pathway; negative regulation of cell migration; negative regulation of epithelial cell proliferation; negative regulation of B cell differentiation; negative regulation of JNK activity; Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; male gonad development; development of primary male sexual characteristics; neural tube closure; negative regulation of cell growth

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

665

size4 :

5x96-Strip-Wells

price4 :

2715

size5 :

10x96-Strip-Wells

price5 :

4895