ELISA/assay

Cytochrome P450 Reductase (CPR) ELISA Kit

MBS2023374

48-Strip-Wells

565 USD

ELISA Kit

Cytochrome P450 Reductase (CPR) ELISA Kit

Cytochrome P450 Reductase (CPR)

POR; CYPOR; P450R; P450(Cytochrome)Oxidoreductase; NADPH--cytochrome P450 reductase

cytochrome P450 reductase, partial; NADPH--cytochrome P450 reductase; NADPH--cytochrome P450 reductase; P450 (cytochrome) oxidoreductase

CPR

POR; POR; CPR; CYPOR; P450R; CYPOR; CPR; P450R

NCPR_HUMAN

Human

677

This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 Reductase (CPR). No significant cross-reactivity or interference between Cytochrome P450 Reductase (CPR) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.059ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 Reductase (CPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 Reductase (CPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 Reductase (CPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cytochrome P450 Reductase (CPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

247307

AAB21814.1

P16435

76,690 Da

Bile Acid Biosynthesis, Neutral Pathway (545316); Bile Acid Biosynthesis, Neutral Pathway (138622); Cytochrome P450 Pathway (198773); Melatonin Degradation I Pathway (545356); Melatonin Degradation I Pathway (545637); Superpathway Of Melatonin Degradation (545640); Superpathway Of Melatonin Degradation (545359); Superpathway Of Tryptophan Utilization (835392); Vitamin D3 Biosynthesis Pathway (545291); Vitamin D3 Biosynthesis Pathway (138457)

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]

POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome. Protein type: EC 1.6.2.4; Oxidoreductase. Chromosomal Location of Human Ortholog: 7q11.2. Cellular Component: endoplasmic reticulum membrane; mitochondrion; membrane; intracellular membrane-bound organelle. Molecular Function: protein binding; enzyme binding; FAD binding; electron carrier activity; NADPH-hemoprotein reductase activity; hydrolase activity; FMN binding; nitric oxide dioxygenase activity; cytochrome-b5 reductase activity; iron ion binding; NADP binding; iron-cytochrome-c reductase activity. Biological Process: response to drug; nitric oxide catabolic process; nitrate catabolic process; positive regulation of chondrocyte differentiation; internal peptidyl-lysine acetylation; flavonoid metabolic process; positive regulation of monooxygenase activity; positive regulation of cholesterol biosynthetic process; positive regulation of smoothened signaling pathway; carnitine metabolic process; fatty acid oxidation; negative regulation of caspase activity; negative regulation of lipase activity; response to nutrient. Disease: Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency; Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340