ELISA/assay

Sorbitol Dehydrogenase (SDH) ELISA Kit

MBS2023295

48-Strip-Wells

575 USD

ELISA Kit

Sorbitol Dehydrogenase (SDH) ELISA Kit

Sorbitol Dehydrogenase (SDH)

SORD; L-iditol 2-dehydrogenase

sorbitol dehydrogenase; Sorbitol dehydrogenase; sorbitol dehydrogenase; sorbitol dehydrogenase; L-iditol 2-dehydrogenase

SDH

SORD; SORD; SORD1; HEL-S-95n

DHSO_HUMAN

Rat

100

This assay has high sensitivity and excellent specificity for detection of Sorbitol Dehydrogenase (SDH). No significant cross-reactivity or interference between Sorbitol Dehydrogenase (SDH) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: Typically less than 0.66ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorbitol Dehydrogenase (SDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorbitol Dehydrogenase (SDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorbitol Dehydrogenase (SDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Sorbitol Dehydrogenase (SDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

156627571

NP_003095.2

NM_003104.5

Q00796

10,812 Da

Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Glucuronate Pathway (uronate Pathway) (997799); Glucuronate Pathway (uronate Pathway) (468207); Metabolic Pathways (132956); Pentose And Glucuronate Interconversions Pathway (82929); Pentose And Glucuronate Interconversions Pathway (290); Polyol Pathway (198815); Sorbitol Degradation I Pathway (142439)

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]

SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family. Protein type: Carbohydrate Metabolism - fructose and mannose; Oxidoreductase; EC 1.1.1.14. Chromosomal Location of Human Ortholog: 15q15.3. Cellular Component: extracellular space; membrane; mitochondrial membrane. Molecular Function: zinc ion binding; L-iditol 2-dehydrogenase activity; carbohydrate binding; NAD binding. Biological Process: sperm motility; L-xylitol metabolic process; L-xylitol catabolic process; glucose metabolic process; fructose biosynthetic process; sorbitol catabolic process

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3065

10x96-Strip-Wells

5510