ELISA/assay

Transforming Growth Factor Alpha (TGFa) ELISA Kit

MBS2023292

48-Strip-Wells

525 USD

ELISA Kit

Transforming Growth Factor Alpha (TGFa) ELISA Kit

Transforming Growth Factor Alpha (TGFa)

ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF

transforming growth factor alpha, partial; Protransforming growth factor alpha; protransforming growth factor alpha; transforming growth factor, alpha; Transforming growth factor alpha; TGF-alpha

TGFa

TGFA; TGFA; TFGA; TGF-alpha; ETGF

TGFA_HUMAN

Mouse

162

This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Alpha (TGFa). No significant cross-reactivity or interference between Transforming Growth Factor Alpha (TGFa) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 7.81-500pg/mL. Sensitivity: Typically less than 3.0pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Alpha (TGFa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Alpha (TGFa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Alpha (TGFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Alpha (TGFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Alpha (TGFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Transforming Growth Factor Alpha (TGFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

553791

AAA79958.1

P01135

17,329 Da

Direct P53 Effectors Pathway (137939); ErbB Receptor Signaling Network Pathway (138016); ErbB Signaling Pathway (198844); ErbB Signaling Pathway (83049); ErbB Signaling Pathway (458); FOXM1 Transcription Factor Network Pathway (137935); Glioma Pathway (83110); Glioma Pathway (522); Non-small Cell Lung Cancer Pathway (83119); Non-small Cell Lung Cancer Pathway (531)

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell cycle regulation; Motility/polarity/chemotaxis; Ligand, receptor tyrosine kinase. Chromosomal Location of Human Ortholog: 2p13. Cellular Component: extracellular space; cell surface; integral to plasma membrane; perinuclear region of cytoplasm; basolateral plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus. Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding. Biological Process: response to drug; epidermal growth factor receptor signaling pathway; cell proliferation; wound healing; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis

48-Strip-Wells

525 USD

96-Strip-Wells

715

5x96-Strip-Wells

2760

10x96-Strip-Wells

4985