ELISA/assay

Histamine Receptor H4 (HRH4) ELISA Kit

MBS2023167

48-Strip-Wells

565 USD

ELISA Kit

Histamine Receptor H4 (HRH4) ELISA Kit

Histamine Receptor H4 (HRH4)

GPCR105; GPRv53; H4R; HH4R; H4; AXOR35; BG26; G-protein coupled receptor 105

histamine H4 receptor isoform 2; Histamine H4 receptor; histamine H4 receptor; histamine receptor H4; AXOR35; G-protein coupled receptor 105; GPRv53; Pfi-013; SP9144

HRH4

HRH4; HRH4; H4; H4R; BG26; HH4R; AXOR35; GPRv53; GPCR105; GPCR105; H4R; HH4R

HRH4_HUMAN

Human

302

This assay has high sensitivity and excellent specificity for detection of Histamine Receptor H4 (HRH4). No significant cross-reactivity or interference between Histamine Receptor H4 (HRH4) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.057ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histamine Receptor H4 (HRH4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histamine Receptor H4 (HRH4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histamine Receptor H4 (HRH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histamine Receptor H4 (HRH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histamine Receptor H4 (HRH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Histamine Receptor H4 (HRH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

219842331

NP_001137300.1

NM_001143828.1

Q9H3N8

34,497 Da

Amine Ligand-binding Receptors Pathway (106363); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Other Pathway (198765); Histamine Receptors Pathway (106367); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637)

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by a family of histamine receptors, which are a subset of the G-protein coupled receptor superfamily. This gene encodes a histamine receptor that is predominantly expressed in haematopoietic cells. The protein is thought to play a role in inflammation and allergy reponses. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

H4R: The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist). Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1. Chromosomal Location of Human Ortholog: 18q11.2. Cellular Component: plasma membrane; integral to membrane. Molecular Function: histamine receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; regulation of MAPKKK cascade; inflammatory response

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340