WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit | MyBioSource MBS2023164 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit

catalog :

MBS2023164

quantity :

24-Strip-Wells

price :

270 USD

product information

catalog number :

MBS2023164

products type :

ELISA Kit

products full name :

WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit

products short name :

[WNT1 Inducible Signaling Pathway Protein 2 (WISP2)]

products name syn :

[CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58]

other names :

[WNT1-inducible-signaling pathway protein 2; WNT1-inducible-signaling pathway protein 2; WNT1-inducible-signaling pathway protein 2; WNT1 inducible signaling pathway protein 2; CCN family member 5; Connective tissue growth factor-like protein; CTGF-L; Connective tissue growth factor-related protein 58]

products gene name :

[WISP2]

other gene names :

[WISP2; WISP2; CCN5; CT58; CTGF-L; CCN5; CT58; CTGFL; WISP-2; CTGF-L]

uniprot entry name :

WISP2_HUMAN

reactivity :

Human

sequence length :

218

specificity :

This assay has high sensitivity and excellent specificity for detection of WISP2. No significant cross-reactivity or interference between WISP2 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Tissue homogenates, cell lysates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.112ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WISP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WISP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Signal transduction

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WISP2 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to WISP2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WISP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WISP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WISP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4507923

ncbi acc num :

NP_003872.1

ncbi gb acc num :

NM_003881.2

uniprot acc num :

O76076

ncbi mol weight :

22,577 Da

ncbi summary :

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. [provided by RefSeq, Jul 2008]

uniprot summary :

WISP2: May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production. Belongs to the CCN family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular space; proteinaceous extracellular matrix; plasma membrane. Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding. Biological Process: cell-cell signaling; regulation of cell growth; cell adhesion; signal transduction

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

665

size4 :

5x96-Strip-Wells

price4 :

2715

size5 :

10x96-Strip-Wells

price5 :

4895