Trichohyalin (TCHH) ELISA Kit | MyBioSource MBS2023135 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Trichohyalin (TCHH) ELISA Kit

catalog :

MBS2023135

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2023135

products type :

ELISA Kit

products full name :

Trichohyalin (TCHH) ELISA Kit

products short name :

Trichohyalin (TCHH)

products name syn :

THH; THL; TRHY

other names :

trichohyalin; Trichohyalin; trichohyalin; trichohyalin

products gene name :

TCHH

other gene names :

TCHH; TCHH; THH; THL; TRHY; THH; THL; TRHY

uniprot entry name :

TRHY_HUMAN

reactivity :

Human

sequence length :

1,943

specificity :

This assay has high sensitivity and excellent specificity for detection of TCHH. No significant cross-reactivity or interference between TCHH and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: Typically less than 0.063ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TCHH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TCHH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TCHH in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TCHH. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TCHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TCHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TCHH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

148746195

ncbi acc num :

NP_009044.2

ncbi gb acc num :

NM_007113.3

uniprot acc num :

Q07283

ncbi mol weight :

253,925 Da

ncbi summary :

Trichohyalin confers mechanical strength to the hair follicle inner root sheath and to other toughened epithelial tissues, such as the hard palate and filiform ridges of the tongue, by forming multiple complex crosslinks with itself and with other structural proteins (Steinert et al., 2003 [PubMed 12853460]).[supplied by OMIM, Dec 2009]

uniprot summary :

THH: Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation. Belongs to the S100-fused protein family. Protein type: Adaptor/scaffold; Calcium-binding. Chromosomal Location of Human Ortholog: 1q21.3. Cellular Component: cytoskeleton. Molecular Function: calcium ion binding. Biological Process: keratinization

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage