Delta Like Protein 4 (dLL4) ELISA Kit | MyBioSource MBS2023134 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Delta Like Protein 4 (dLL4) ELISA Kit

catalog :

MBS2023134

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2023134

products type :

ELISA Kit

products full name :

Delta Like Protein 4 (dLL4) ELISA Kit

products short name :

Delta Like Protein 4 (dLL4)

products name syn :

Hdelta2; Drosophila Delta homolog 4

other names :

delta-like protein 4; Delta-like protein 4; delta-like protein 4; delta-like 4 (Drosophila); Drosophila Delta homolog 4; Delta4

products gene name :

dLL4

other gene names :

DLL4; DLL4; hdelta2; Delta4

uniprot entry name :

DLL4_HUMAN

reactivity :

Human

sequence length :

685

specificity :

This assay has high sensitivity and excellent specificity for detection of dLL4. No significant cross-reactivity or interference between dLL4 and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: 0.054ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level dLL4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level dLL4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of dLL4 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to dLL4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to dLL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain dLL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of dLL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

9506545

ncbi acc num :

NP_061947.1

ncbi gb acc num :

NM_019074.3

uniprot acc num :

Q9NR61

ncbi mol weight :

74,605 Da

ncbi pathways :

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway (771591); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (771593); Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway (771589); Delta-Notch Signaling Pathway (198879); Disease Pathway (530764); FBXW7 Mutants And NOTCH1 In Cancer Pathway (771596); NOTCH2 Activation And Transmission Of Signal To The Nucleus Pathway (771602); Neural Crest Differentiation Pathway (672460)

ncbi summary :

This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq, Jul 2008]

uniprot summary :

DLL4: Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4. Binds to Notch-1 and Notch-4. Expressed in vascular endothelium. Protein type: Membrane protein, integral; Cell development/differentiation. Chromosomal Location of Human Ortholog: 15q14. Cellular Component: integral to membrane; plasma membrane. Molecular Function: calcium ion binding; Notch binding. Biological Process: patterning of blood vessels; negative regulation of cell proliferation; Notch signaling pathway; regulation of neurogenesis; visual perception; blood circulation; Notch receptor processing; blood vessel remodeling; angiogenesis; negative regulation of transcription from RNA polymerase II promoter; signal transduction

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage