Sulfatase 2 (SULF2) ELISA Kit | MyBioSource MBS2023117 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Sulfatase 2 (SULF2) ELISA Kit

catalog :

MBS2023117

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2023117

products type :

ELISA Kit

products full name :

Sulfatase 2 (SULF2) ELISA Kit

products short name :

Sulfatase 2 (SULF2)

products name syn :

HSULF-2; Extracellular sulfatase Sulf-2

other names :

Sulfatase 2; Extracellular sulfatase Sulf-2; extracellular sulfatase Sulf-2; sulfatase 2

products gene name :

SULF2

other gene names :

SULF2; SULF2; HSULF-2; KIAA1247; hSulf-2

uniprot entry name :

SULF2_HUMAN

reactivity :

Human

sequence length :

867

specificity :

This assay has high sensitivity and excellent specificity for detection of SULF2. No significant cross-reactivity or interference between SULF2 and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.312-20ng/mL. Sensitivity: The minimum detectable dose of SULF2 is typically less than 0.121ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SULF2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SULF2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SULF2 in human serum, plasma, tissue homogenates and other biological fluids.

products description :

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SULF2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SULF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SULF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SULF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

83405533

ncbi acc num :

AAI10540.1

uniprot acc num :

Q8IWU5

ncbi mol weight :

100,156 Da

ncbi summary :

Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM, Mar 2008]

uniprot summary :

SULF2: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; EC 3.1.6.-; Hydrolase; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular space; Golgi stack; cell surface; endoplasmic reticulum; plasma membrane. Molecular Function: arylsulfatase activity; N-acetylglucosamine-6-sulfatase activity; calcium ion binding. Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; cartilage development; embryonic skeletal development; glomerular basement membrane development; esophagus smooth muscle contraction; glomerular filtration; heparan sulfate proteoglycan metabolic process; chondrocyte development; kidney development; positive regulation of Wnt receptor signaling pathway

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage