ELISA/assay

Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit

MBS2023115

48-Strip-Wells

515 USD

ELISA Kit

Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit

Superoxide Dismutase 3, Extracellular (SOD3)

SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn

superoxide dismutase 3, extracellular; Extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; superoxide dismutase 3, extracellular

SOD3

SOD3; SOD3; EC-SOD; EC-SOD

SODE_HUMAN

Human

240

This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 3, Extracellular (SOD3). No significant cross-reactivity or interference between Superoxide Dismutase 3, Extracellular (SOD3) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 125-8000pg/mL. Sensitivity: Typically less than 50pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase 3, Extracellular (SOD3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Superoxide Dismutase 3, Extracellular (SOD3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119613229

EAW92823.1

P08294

25,851 Da

Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (1127552); Folate Metabolism Pathway (198833); Oxidative Stress Pathway (198916); Selenium Pathway (198825); Vitamin B12 Metabolism Pathway (920974); Reactive Oxygen Species Degradation Pathway (1108784); Reactive Oxygen Species Degradation (mammalian) Pathway (1108902)

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM. [provided by RefSeq, Jul 2008]

SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family. Protein type: Secreted, signal peptide; Secreted; EC 1.15.1.1; Oxidoreductase. Chromosomal Location of Human Ortholog: 4p15.2. Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus. Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity. Biological Process: response to reactive oxygen species; removal of superoxide radicals; response to copper ion; response to hypoxia

48-Strip-Wells

515 USD

96-Strip-Wells

695

5x96-Strip-Wells

2685

10x96-Strip-Wells

4850