ELISA/assay

L1-Cell Adhesion Molecule (L1CAM) ELISA Kit

MBS2023094

48-Strip-Wells

515 USD

ELISA Kit

L1-Cell Adhesion Molecule (L1CAM) ELISA Kit

L1-Cell Adhesion Molecule (L1CAM)

CD171; L1-CAM; CAML1; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; Antigen Identified By Monoclonal Antibody R1

L1 cell adhesion molecule; Neural cell adhesion molecule L1; neural cell adhesion molecule L1; L1 cell adhesion molecule; CD_antigen: CD171

L1CAM

L1CAM; L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1; CAML1; MIC5; N-CAM-L1; NCAM-L1

L1CAM_HUMAN

Human

1,248

This assay has high sensitivity and excellent specificity for detection of L1-Cell Adhesion Molecule (L1CAM). No significant cross-reactivity or interference between L1-Cell Adhesion Molecule (L1CAM) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 6.7pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level L1-Cell Adhesion Molecule (L1CAM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level L1-Cell Adhesion Molecule (L1CAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L1-Cell Adhesion Molecule (L1CAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to L1-Cell Adhesion Molecule (L1CAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L1-Cell Adhesion Molecule (L1CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of L1-Cell Adhesion Molecule (L1CAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

187951475

AAI36448.1

P32004

138,908 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Basigin Interactions Pathway (106065); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Developmental Biology Pathway (477129); Hemostasis Pathway (106028); Interaction Between L1 And Ankyrins Pathway (161005)

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: presynaptic membrane; focal adhesion; cell surface; plasma membrane; integral to membrane; terminal button; external side of plasma membrane. Molecular Function: integrin binding; identical protein binding; protein self-association; sialic acid binding. Biological Process: heterophilic cell adhesion; nervous system development; axon guidance; leukocyte adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; cell-cell adhesion mediated by integrin; positive regulation of cell-cell adhesion; positive regulation of calcium-mediated signaling; homophilic cell adhesion; cell adhesion; chemotaxis; blood coagulation; leukocyte migration. Disease: Hydrocephalus Due To Congenital Stenosis Of Aqueduct Of Sylvius; Corpus Callosum, Partial Agenesis Of, X-linked; Masa Syndrome

48-Strip-Wells

515 USD

96-Strip-Wells

695

5x96-Strip-Wells

2685

10x96-Strip-Wells

4850