Endoglin (ENG) ELISA Kit | MyBioSource MBS2023081 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Endoglin (ENG) ELISA Kit

catalog :

MBS2023081

quantity :

48-Strip-Wells

price :

550 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2023081

products type :

ELISA Kit

products full name :

Endoglin (ENG) ELISA Kit

products short name :

Endoglin (ENG)

products name syn :

CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1

other names :

endoglin isoform 1; Endoglin; endoglin; endoglin; CD_antigen: CD105

products gene name :

ENG

other gene names :

ENG; ENG; END; HHT1; ORW1; END

uniprot entry name :

EGLN_HUMAN

reactivity :

Rat

sequence length :

625

specificity :

This assay has high sensitivity and excellent specificity for detection of endoglin. No significant cross-reactivity or interference between endoglin and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Rat serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.78 -50ng/mL. The standard curve concentrations used for the ELISA's were 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.12ng/mL, 1.56ng/mL, 0.78ng/mL. Sensitivity: 0.35ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level endoglin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level endoglin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of endoglin in rat serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to endoglin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to endoglin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain endoglin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of endoglin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

168693647

ncbi acc num :

NP_001108225.1

ncbi gb acc num :

NM_001114753.2

uniprot acc num :

P17813

ncbi mol weight :

67,542 Da

ncbi pathways :

HIF-1-alpha Transcription Factor Network Pathway (138045); TGF Beta Signaling Pathway (198810); TGF-beta Receptor Signaling Pathway (198774)

ncbi summary :

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

uniprot summary :

ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 9q34.11. Cellular Component: nucleoplasm; extracellular space; focal adhesion; cell surface; cytoplasm; receptor complex; external side of plasma membrane. Molecular Function: transforming growth factor beta receptor activity; protein binding; transmembrane receptor activity; glycosaminoglycan binding; protein homodimerization activity; transforming growth factor beta binding; galactose binding; punt binding; activin binding; transforming growth factor beta receptor, cytoplasmic mediator activity. Biological Process: wound healing; positive regulation of collagen biosynthetic process; cell motility involved in cell locomotion; positive regulation of systemic arterial blood pressure; negative regulation of transcription from RNA polymerase II promoter; response to corticosteroid stimulus; BMP signaling pathway; smooth muscle development; extracellular matrix disassembly; central nervous system vasculogenesis; regulation of transforming growth factor beta receptor signaling pathway; venous blood vessel morphogenesis; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; heart looping; cell adhesion; chronological cell aging; vasculogenesis; negative regulation of cell migration; positive regulation of BMP signaling pathway; regulation of cell adhesion; cell migration; negative regulation of nitric-oxide synthase activity; regulation of phosphorylation; regulation of cell proliferation; patterning of blood vessels; negative regulation of endothelial cell proliferation; response to hypoxia; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway. Disease: Telangiectasia, Hereditary Hemorrhagic, Of Rendu, Osler, And Weber

size1 :

48-Strip-Wells

price1 :

550 USD

size2 :

96-Strip-Wells

price2 :

750

size3 :

5x96-Strip-Wells

price3 :

2910

size4 :

10x96-Strip-Wells

price4 :

5220

more info or order :

MyBioSource product webpage