ELISA/assay

C-Terminal Binding Protein 2 (CTBP2) ELISA Kit

MBS2023074

96 Strip Wells

780 USD

ELISA Kit

C-Terminal Binding Protein 2 (CTBP2) ELISA Kit

C-Terminal Binding Protein 2 (CTBP2)

Ribeye

C-terminal binding protein 2; C-terminal-binding protein 2; C-terminal-binding protein 2; C-terminal binding protein 2

CTBP2

CTBP2; CTBP2; CtBP2

CTBP2_HUMAN

Human

985

This assay has high sensitivity and excellent specificity for detection of C-Terminal Binding Protein 2 (CTBP2). No significant cross-reactivity or interference between C-Terminal Binding Protein 2 (CTBP2) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Tissue homogenates and other biological fluids. Detection Range: 0.313-20ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 0.125ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Terminal Binding Protein 2 (CTBP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Terminal Binding Protein 2 (CTBP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Terminal Binding Protein 2 (CTBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Terminal Binding Protein 2 (CTBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Terminal Binding Protein 2 (CTBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of C-Terminal Binding Protein 2 (CTBP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

2909777

AAC39603.1

P56545

106,187 Da

AMER1 Mutants Destabilize The Destruction Complex Pathway 1127621!!APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway 1127616!!APC Truncation Mutants Have Impaired AXIN Binding Pathway 1127617!!AXIN Missense Mutants Destabilize The Destruction Complex Pathway 1127620!!AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway 1127618!!Chronic Myeloid Leukemia Pathway 83116!!Chronic Myeloid Leukemia Pathway 528!!Degradation Of Beta-catenin By The Destruction Complex Pathway 106511!!Disease Pathway 530764!!Neural Crest Differentiation Pathway 672460

This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]

96 Strip Wells

780 USD