ELISA/assay

Aromatase (ARO) ELISA Kit

MBS2023052

24-Strip-Wells

285 USD

ELISA Kit

Aromatase (ARO) ELISA Kit

[Aromatase (ARO)]

[CYP19A1; CYPXIX; ARO; ARO1; CPV1; CYAR; CYP19; P450AROM; P450-AROM; Estrogen synthase; Cytochrome P450,Family 19,Subfamily A,Polypeptide 1]

[aromatase; Aromatase; aromatase; cytochrome P450, family 19, subfamily A, polypeptide 1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase]

[ARO]

[CYP19A1; CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM; ARO1; CYAR; CYP19]

CP19A_HUMAN

Rat

218

This assay has high sensitivity and excellent specificity for detection of Aromatase (ARO). No significant cross-reactivity or interference between Aromatase (ARO) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids. Detection Range: 1.56-100ng/mL. Sensitivity: < 0.62ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aromatase (ARO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aromatase (ARO) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Enzyme Tumor immunity; Endocrinology; Hormone metabolism

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aromatase (ARO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aromatase (ARO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase (ARO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Aromatase (ARO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

13904860

NP_112503.1

NM_031226.2

P11511

24,518 Da

Biological Oxidations Pathway (105698); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (413383); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (468268); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway (1127640); Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway (1127641); Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway (1127642); Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway (1127643); Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway (1127644); Defective CYP1B1 Causes Glaucoma Pathway (1127645)

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family. Protein type: Oxidoreductase; Lipid Metabolism - androgen and estrogen; EC 1.14.14.14. Chromosomal Location of Human Ortholog: 15q21.1. Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum. Molecular Function: electron carrier activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; oxygen binding. Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; sterol metabolic process; estrogen biosynthetic process; steroid biosynthetic process. Disease: Aromatase Deficiency; Aromatase Excess Syndrome

24-Strip-Wells

285 USD

48-Strip-Wells

530

96-Strip-Wells

715

5x96-Strip-Wells

2910

10x96-Strip-Wells

5280