ELISA/assay

Teratocarcinoma Derived Growth Factor 1 (TDGF1) ELISA Kit

MBS2023019

48-Strip-Wells

565 USD

ELISA Kit

Teratocarcinoma Derived Growth Factor 1 (TDGF1) ELISA Kit

Teratocarcinoma Derived Growth Factor 1 (TDGF1)

CR; CRGF; CRIPTO; Cripto-1; Epidermal growth factor-like cripto protein CR1; Cripto-1 growth factor

Teratocarcinoma-derived growth factor 1; Teratocarcinoma-derived growth factor 1; teratocarcinoma-derived growth factor 1; teratocarcinoma-derived growth factor 1; Cripto-1 growth factor; CRGF; Epidermal growth factor-like cripto protein CR1

TDGF1

TDGF1; TDGF1; CR; CRGF; CRIPTO; CRIPTO; CRGF

TDGF1_HUMAN

Human

188

This assay has high sensitivity and excellent specificity for detection of TDGF1. No significant cross-reactivity or interference between TDGF1 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human serum, plasma, tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 78-5,000pg/mL. The standard curve concentrations used for the ELISA's were 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL. Sensitivity: 28pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TDGF1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TDGF1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TDGF1 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to TDGF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TDGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TDGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TDGF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

117473

P13385.1

P13385

21,169 Da

Developmental Biology Pathway (477129); Glypican 1 Network Pathway (138010); POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway (1127697); Regulation Of Signaling By NODAL Pathway (477131); Signaling By NODAL Pathway (477130); Transcriptional Regulation Of Pluripotent Stem Cells Pathway (1127696)

This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

TDGF1: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Interacts with the activin type-1 receptor ACVR1B. Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung. Protein type: Membrane protein, GPI anchor; Membrane protein, peripheral; Motility/polarity/chemotaxis; Cell adhesion; Cell cycle regulation. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: extrinsic to plasma membrane; Golgi apparatus; extracellular space; cell surface; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; nucleus; lipid raft. Molecular Function: protein binding; growth factor activity; receptor binding. Biological Process: activation of MAPK activity; somatic stem cell maintenance; heart development; regulation of signal transduction; gastrulation; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; positive regulation of fibroblast proliferation; mammary gland development; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; vasculogenesis; positive regulation of cell-matrix adhesion; cell differentiation; epidermal growth factor receptor signaling pathway; in utero embryonic development; morphogenesis of a branching structure; embryonic development; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of apoptosis; positive regulation of cell migration

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340