ELISA/assay

Cardiotrophin Like Cytokine Factor 1 (CLCF1) ELISA Kit

MBS2023014

48-Strip-Wells

575 USD

ELISA Kit

Cardiotrophin Like Cytokine Factor 1 (CLCF1) ELISA Kit

Cardiotrophin Like Cytokine Factor 1 (CLCF1)

CLC; NR6; BSF3; CISS2; NNT1; Novel Neurotrophin-1; B Cell-Stimulating Factor-3

cardiotrophin-like cytokine factor 1 isoform 1; Cardiotrophin-like cytokine factor 1; cardiotrophin-like cytokine factor 1; cardiotrophin-like cytokine factor 1; B-cell-stimulating factor 3; BSF-3; Novel neurotrophin-1; NNT-1

CLCF1

Clcf1; Clcf1; CLC; Bsf3; BSF-3; NNT-1; Bsf3; Nnt1; BSF-3; NNT-1

CLCF1_MOUSE

Mouse

225

This assay has high sensitivity and excellent specificity for detection of Cardiotrophin Like Cytokine Factor 1 (CLCF1). No significant cross-reactivity or interference between Cardiotrophin Like Cytokine Factor 1 (CLCF1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 6.4pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiotrophin Like Cytokine Factor 1 (CLCF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiotrophin Like Cytokine Factor 1 (CLCF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cardiotrophin Like Cytokine Factor 1 (CLCF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cardiotrophin Like Cytokine Factor 1 (CLCF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

9910314

NP_064336.1

NM_019952.5

25,261 Da

Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83274); Jak-STAT Signaling Pathway (488)

CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Apoptosis; Secreted; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region; intracellular. Molecular Function: protein binding; protein heterodimerization activity; cytokine activity; ciliary neurotrophic factor receptor binding; receptor binding. Biological Process: positive regulation of T-helper 2 type immune response; regulation of protein kinase B signaling cascade; JAK-STAT cascade; positive regulation of isotype switching to IgE isotypes; positive regulation of corticotropin-releasing hormone secretion; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of immunoglobulin production; cell surface receptor linked signal transduction; B cell differentiation; positive regulation of cell proliferation; regulation of inflammatory response; positive regulation of B cell proliferation; negative regulation of neuron apoptosis; positive regulation of astrocyte differentiation

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3055

10x96-Strip-Wells

5495