Semaphorin 4D (SEMA4D) ELISA Kit | MyBioSource MBS2023012 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Semaphorin 4D (SEMA4D) ELISA Kit

catalog :

MBS2023012

quantity :

24-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2023012

products type :

ELISA Kit

products full name :

Semaphorin 4D (SEMA4D) ELISA Kit

products short name :

[Semaphorin 4D (SEMA4D)]

products name syn :

[CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D]

other names :

[semaphorin-4D isoform 1; Semaphorin-4D; semaphorin-4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; A8; BB18; GR3; CD_antigen: CD100]

products gene name :

[SEMA4D]

other gene names :

[SEMA4D; SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G; C9orf164; CD100; SEMAJ]

uniprot entry name :

SEM4D_HUMAN

reactivity :

Human

sequence length :

862

specificity :

This assay has high sensitivity and excellent specificity for detection of SEMA3B. No significant cross-reactivity or interference between SEMA3B and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.055ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA3B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA3B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA3B in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SEMA3B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SEMA3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SEMA3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

214010218

ncbi acc num :

NP_006369.3

ncbi gb acc num :

NM_006378.3

ncbi mol weight :

82,164 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Other Semaphorin Interactions Pathway (119526); Sema4D In Semaphorin Signaling Pathway (119520); Sema4D Induced Cell Migration And Growth-cone Collapse Pathway (119522); Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway (119521); Semaphorin Interactions Pathway (119519)

uniprot summary :

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 9q22.2. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane. Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding. Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; axon guidance; regulation of dendrite morphogenesis; negative regulation of cell adhesion; negative regulation of transcription from RNA polymerase II promoter; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; immune response; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; negative regulation of apoptosis; regulation of cell projection organization and biogenesis; positive regulation of cell migration

size1 :

24-Strip-Wells

price1 :

260 USD

size2 :

48-Strip-Wells

price2 :

475

size3 :

96-Strip-Wells

price3 :

640

size4 :

5x96-Strip-Wells

price4 :

2590

size5 :

10x96-Strip-Wells

price5 :

4670

more info or order :

MyBioSource product webpage