ELISA/assay

Integrin Alpha 1 (ITGa1) ELISA Kit

MBS2022992

48-Strip-Wells

540 USD

ELISA Kit

Integrin Alpha 1 (ITGa1) ELISA Kit

Integrin Alpha 1 (ITGa1)

CD49-A; CD49a; ITG-A1; VLA1; CD49 antigen-like family member A; Laminin and collagen receptor

integrin alpha-1; Integrin alpha-1; integrin alpha-1; integrin, alpha 1; CD49 antigen-like family member A; Laminin and collagen receptor; VLA-1; CD_antigen: CD49a

ITGa1

ITGA1; ITGA1; VLA1; CD49a

ITA1_HUMAN

Human

1,179

This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 1 (ITGa1). No significant cross-reactivity or interference between Integrin Alpha 1 (ITGa1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: Typically less than 29pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Alpha 1 (ITGa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Alpha 1 (ITGa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 1 (ITGa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 1 (ITGa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 1 (ITGa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Integrin Alpha 1 (ITGa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

31657142

NP_852478.1

NM_181501.1

P56199

130,848 Da

Arf6 Trafficking Events Pathway (137954); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (105688); CHL1 Interactions Pathway (161007); CXCR4-mediated Signaling Events Pathway (137910); Developmental Biology Pathway (477129); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285)

This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]

ITGA1: Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen. Belongs to the integrin alpha chain family. Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 5q11.2. Cellular Component: neuron projection; cell surface; focal adhesion; membrane; acrosome; plasma membrane; perikaryon; integrin complex; external side of plasma membrane; lipid raft. Molecular Function: collagen binding; protein binding; metal ion binding; protein phosphatase binding; receptor binding. Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; neutrophil chemotaxis; axon guidance; extracellular matrix organization and biogenesis; activation of MAPK activity; cell-matrix adhesion; vasodilation; negative regulation of cell proliferation; muscle contraction; cellular extravasation; positive regulation of neuron apoptosis; blood coagulation

48-Strip-Wells

540 USD

96-Strip-Wells

730

5x96-Strip-Wells

2830

10x96-Strip-Wells

5080