ELISA/assay

Collagen Type IV (COL4) ELISA Kit

MBS2022979

24-Strip-Wells

255 USD

ELISA Kit

Collagen Type IV (COL4) ELISA Kit

[Collagen Type IV (COL4)]

[Type-IV Collagen]

[collagen type IV alpha 5 chain, partial; Collagen alpha-5(IV) chain; collagen alpha-5(IV) chain; collagen, type IV, alpha 5]

[COL4]

[COL4A5; COL4A5; ATS; ASLN; CA54]

CO4A5_HUMAN

Human

1,691

This assay has high sensitivity and excellent specificity for detection of COL3. No significant cross-reactivity or interference between COL3 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.12-200ng/mL. Sensitivity: 1.22ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Tumor immunity; Hepatology

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL3 in rat serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4261609

AAD13909.1

161,632 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Axon Guidance Pathway (105688); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Developmental Biology Pathway (477129); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262)

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis; Secreted. Chromosomal Location of Human Ortholog: Xq22. Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region; basal lamina; neuromuscular junction. Molecular Function: extracellular matrix structural constituent. Biological Process: extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; neuromuscular junction development. Disease: Alport Syndrome, X-linked

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445