Insulin Like Growth Factor 1 (IGF1) ELISA Kit | MyBioSource MBS2022910 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Insulin Like Growth Factor 1 (IGF1) ELISA Kit

catalog :

MBS2022910

quantity :

48-Strip-Wells

price :

600 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022910

products type :

ELISA Kit

products full name :

Insulin Like Growth Factor 1 (IGF1) ELISA Kit

products short name :

Insulin Like Growth Factor 1 (IGF1)

products name syn :

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

other names :

insulin-like growth factor I preproprotein; Insulin-like growth factor I; insulin-like growth factor I; Somatomedin

products gene name :

IGF1

other gene names :

IGF1; IGF1; IGF-1; IGF-I; IGF-I

uniprot entry name :

IGF1_BOVIN

reactivity :

Bovine

sequence length :

154

specificity :

This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1). No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: Typically less than 27.3pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 (IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Insulin Like Growth Factor 1 (IGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

118150782

ncbi acc num :

NP_001071296.1

ncbi gb acc num :

NM_001077828.1

uniprot acc num :

P07455

ncbi mol weight :

17,066 Da

ncbi pathways :

AMPK Signaling Pathway (989258); AMPK Signaling Pathway (992181); Adipogenesis Pathway (920913); Aldosterone-regulated Sodium Reabsorption Pathway (130625); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Androgen Receptor Signaling Pathway (198553); Apoptosis Pathway (198572); Dilated Cardiomyopathy Pathway (121480); Dilated Cardiomyopathy Pathway (121285); Endochondral Ossification Pathway (920938)

size1 :

48-Strip-Wells

price1 :

600 USD

size2 :

96-Strip-Wells

price2 :

820

size3 :

5x96-Strip-Wells

price3 :

3205

size4 :

10x96-Strip-Wells

price4 :

5765

more info or order :

MyBioSource product webpage