ELISA/assay

Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit

MBS2022870

24-Strip-Wells

270 USD

ELISA Kit

Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit

[Spermidine/Spermine N1-Acetyltransferase 1 (SAT1)]

[DC21; KFSD; SAT; SSAT; SSAT-1; Diamine Acetyltransferase 1; Polyamine N-acetyltransferase 1; Putrescine acetyltransferase]

[Spermidine/spermine N1-acetyltransferase 1; Diamine acetyltransferase 1; diamine acetyltransferase 1; spermidine/spermine N1-acetyltransferase 1; Polyamine N-acetyltransferase 1; Putrescine acetyltransferase; Spermidine/spermine N(1)-acetyltransferase 1; SSAT; SSAT-1]

[SAT1]

[SAT1; SAT1; SAT; DC21; KFSD; SSAT; KFSDX; SSAT-1; SAT; SSAT; SSAT-1]

SAT1_HUMAN

Human

171

This assay has high sensitivity and excellent specificity for detection of Spermidine/Spermine N1-Acetyltransferase 1 (SAT1). No significant cross-reactivity or interference between Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Standard Curve/Testing Data

Assay Type: Double-antibody Sandwich. Samples: Tissue homogenates, Cell lysates and Other Biological Fluids. Detection Range: 0.78-50ng/mL. Sensitivity: < 0.28ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Enzyme & Kinase

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spermidine/Spermine N1-Acetyltransferase 1 (SAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spermidine/Spermine N1-Acetyltransferase 1 (SAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spermidine/Spermine N1-Acetyltransferase 1 (SAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

14250050

AAH08424.1

P21673

20,024 Da

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); GABA Biosynthesis, Eukaryotes, Putrescine = GABA Pathway (413399); GABA Biosynthesis, Eukaryotes, Putrescine = GABA Pathway (468314); Interconversion Of Polyamines Pathway (106195); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Metabolism Of Polyamines Pathway (106193); Nucleotide Metabolism Pathway (198876)

The protein encoded by this gene belongs to the acetyltransferase family, and is a rate-limiting enzyme in the catabolic pathway of polyamine metabolism. It catalyzes the acetylation of spermidine and spermine, and is involved in the regulation of the intracellular concentration of polyamines and their transport out of cells. Defects in this gene are associated with keratosis follicularis spinulosa decalvans (KFSD). Alternatively spliced transcripts have been found for this gene.[provided by RefSeq, Sep 2009]

SAT: Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine spermine N(1)-acetylspermine putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine. Defects in SAT1 may be a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX). A rare disorder affecting the skin and the eye. Affected men show thickening of the skin of the neck, ears, and extremities, especially the palms and soles, loss of eyebrows, eyelashes and beard, thickening of the eyelids with blepharitis and ectropion, and corneal degeneration. Belongs to the acetyltransferase family. Protein type: Amino Acid Metabolism - arginine and proline; Acetyltransferase; EC 2.3.1.57. Chromosomal Location of Human Ortholog: Xp22.1. Cellular Component: intracellular; cytosol. Molecular Function: protein binding; diamine N-acetyltransferase activity; spermidine binding. Biological Process: spermidine acetylation; polyamine biosynthetic process; putrescine catabolic process; angiogenesis; polyamine metabolic process; regulation of cell proliferation. Disease: Keratosis Follicularis Spinulosa Decalvans, X-linked

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895