VGF Nerve Growth Factor Inducible (VGF) ELISA Kit | MyBioSource MBS2022810 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

VGF Nerve Growth Factor Inducible (VGF) ELISA Kit

catalog :

MBS2022810

quantity :

24-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2022810

products type :

ELISA Kit

products full name :

VGF Nerve Growth Factor Inducible (VGF) ELISA Kit

products short name :

[VGF Nerve Growth Factor Inducible (VGF)]

products name syn :

[NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide]

other names :

[VGF nerve growth factor inducible; Neurosecretory protein VGF; neurosecretory protein VGF; VGF nerve growth factor inducible]

products gene name :

[VGF]

other gene names :

[VGF; VGF; SCG7; SgVII; NERP-1; NERP-2]

uniprot entry name :

VGF_HUMAN

reactivity :

Human

sequence length :

615

specificity :

This assay has high sensitivity and excellent specificity for detection of VGF Nerve Growth Factor Inducible (VGF). No significant cross-reactivity or interference between VGF Nerve Growth Factor Inducible (VGF) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Cerebrospinal fluid and Other Biological Fluids. Detection Range: 78-5,000pg/mL. Sensitivity: < 28pg/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Metabolic pathway; Infection immunity; Neuroscience

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VGF Nerve Growth Factor Inducible (VGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VGF Nerve Growth Factor Inducible (VGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VGF Nerve Growth Factor Inducible (VGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of VGF Nerve Growth Factor Inducible (VGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119570586

ncbi acc num :

EAW50201.1

uniprot acc num :

O15240

ncbi mol weight :

67,258 Da

ncbi summary :

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

uniprot summary :

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q22.1. Cellular Component: extracellular space; transport vesicle; cytoplasmic vesicle. Molecular Function: neuropeptide hormone activity; growth factor activity. Biological Process: response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; sexual reproduction; insulin secretion; defense response to bacterium; response to cold; glucose homeostasis; response to insulin stimulus

size1 :

24-Strip-Wells

price1 :

260 USD

size2 :

48-Strip-Wells

price2 :

475

size3 :

96-Strip-Wells

price3 :

640

size4 :

5x96-Strip-Wells

price4 :

2590

size5 :

10x96-Strip-Wells

price5 :

4670

more info or order :

MyBioSource product webpage