ELISA/assay

A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) ELISA Kit

MBS2022801

48-Strip-Wells

565 USD

ELISA Kit

A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) ELISA Kit

A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

ADMP-1; Aggrecanase-1

A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 4; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADMP-1; Aggrecanase-1

ADAMTS4

ADAMTS4; ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4; KIAA0688; ADAM-TS 4; ADAM-TS4; ADAMTS-4

ATS4_HUMAN

Human

777

This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4). No significant cross-reactivity or interference between A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.134ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

157427675

NP_005090.3

NM_005099.4

O75173

83,030 Da

Degradation Of The Extracellular Matrix Pathway (576263); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (1127680); O-linked Glycosylation Pathway (1127679); Post-translational Protein Modification Pathway (161012)

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008]

ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the 392-Glu- -Ala-393 site. Protein type: Motility/polarity/chemotaxis; EC 3.4.24.82; Secreted, signal peptide; Secreted; Protease. Chromosomal Location of Human Ortholog: 1q21-q23. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: peptidase activity; protein binding; protease binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response to bacterium; proteolysis; skeletal development

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340