Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit | MyBioSource MBS2022788 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit

catalog :

MBS2022788

quantity :

48-Strip-Wells

price :

605 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022788

products type :

ELISA Kit

products full name :

Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit

products short name :

Glutamate Cysteine Ligase, Catalytic (GCLC)

products name syn :

GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECS

other names :

Glutamate-cysteine ligase catalytic subunit, isoform B; Glutamate--cysteine ligase; CG2259 gene product from transcript CG2259-RC; Glutamate-cysteine ligase catalytic subunit; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

products gene name :

GCLC

other gene names :

Gclc; Gclc; 2259; CG2259; DmelCG2259; DmGCLC; DmGCS; DmGCSh; GCL; gclc; GCLc; Gcs; Gcsh; GCSh; GCSh; GCS

uniprot entry name :

GSH1_DROME

reactivity :

Rat

sequence length :

717

specificity :

This assay has high sensitivity and excellent specificity for detection of Glutamate Cysteine Ligase, Catalytic (GCLC). No significant cross-reactivity or interference between Glutamate Cysteine Ligase, Catalytic (GCLC) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.126ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Catalytic (GCLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Catalytic (GCLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, Catalytic (GCLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, Catalytic (GCLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Cysteine Ligase, Catalytic (GCLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Glutamate Cysteine Ligase, Catalytic (GCLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

45554453

ncbi acc num :

NP_996373.1

ncbi gb acc num :

NM_206650.2

uniprot acc num :

Q9W3K5

ncbi mol weight :

80,746 Da

ncbi pathways :

Biological Oxidations Pathway (1116531); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1116772); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1116791); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1116792); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1116793); Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway (1116796); Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway (1116794); Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway (1116798); Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway (1116799); Defective UGT1A1 Causes Hyperbilirubinemia Pathway (1116800)

size1 :

48-Strip-Wells

price1 :

605 USD

size2 :

96-Strip-Wells

price2 :

820

size3 :

5x96-Strip-Wells

price3 :

3220

size4 :

10x96-Strip-Wells

price4 :

5795

more info or order :

MyBioSource product webpage