Surfactant Associated Protein D (SPD) ELISA Kit | MyBioSource MBS2022740 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Surfactant Associated Protein D (SPD) ELISA Kit

catalog :

MBS2022740

quantity :

48-Strip-Wells

price :

550 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022740

products type :

ELISA Kit

products full name :

Surfactant Associated Protein D (SPD) ELISA Kit

products short name :

Surfactant Associated Protein D (SPD)

products name syn :

SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D

other names :

Surfactant associated protein D; Pulmonary surfactant-associated protein D; pulmonary surfactant-associated protein D; surfactant associated protein D; Lung surfactant protein D

products gene name :

SPD

other gene names :

Sftpd; Sftpd; SP-D; Sftp4; AI573415; Sftp4; PSP-D; SP-D

uniprot entry name :

SFTPD_MOUSE

reactivity :

Mouse

sequence length :

374

specificity :

This assay has high sensitivity and excellent specificity for detection of Surfactant Associated Protein D (SPD). No significant cross-reactivity or interference between Surfactant Associated Protein D (SPD) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates, lung lavage fluid and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Typically less than 13.5pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Surfactant Associated Protein D (SPD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Surfactant Associated Protein D (SPD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Associated Protein D (SPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Surfactant Associated Protein D (SPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Associated Protein D (SPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Surfactant Associated Protein D (SPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

13277590

ncbi acc num :

AAH03705.1

uniprot acc num :

P50404

ncbi mol weight :

37,688 Da

ncbi pathways :

Phagosome Pathway (153914); Phagosome Pathway (153859)

uniprot summary :

SFTPD: Contributes to the lung s defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: multivesicular body; proteinaceous extracellular matrix; extracellular space; collagen; rough endoplasmic reticulum; cytoplasm; extracellular region; cytoplasmic vesicle. Molecular Function: monosaccharide binding; identical protein binding; lipopolysaccharide binding; carbohydrate binding. Biological Process: negative regulation of phagocytosis; negative regulation of T cell proliferation; immune system process; positive regulation of phagocytosis; innate immune response; regulation of liquid surface tension; opsonization; respiratory gaseous exchange; alveolus development; surfactant homeostasis; negative regulation of interleukin-2 production

size1 :

48-Strip-Wells

price1 :

550 USD

size2 :

96-Strip-Wells

price2 :

750

size3 :

5x96-Strip-Wells

price3 :

2910

size4 :

10x96-Strip-Wells

price4 :

5220

more info or order :

MyBioSource product webpage