Attractin (ATRN) ELISA Kit | MyBioSource MBS2022736 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Attractin (ATRN) ELISA Kit

catalog :

MBS2022736

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022736

products type :

ELISA Kit

products full name :

Attractin (ATRN) ELISA Kit

products short name :

Attractin (ATRN)

products name syn :

DPPT-L; MGCA; Mahogany homolog

other names :

Attractin; Attractin; attractin; attractin; DPPT-L; Mahogany homolog

products gene name :

ATRN

other gene names :

ATRN; ATRN; MGCA; DPPT-L; KIAA0548; MGCA

uniprot entry name :

ATRN_HUMAN

reactivity :

Human

sequence length :

1,198

specificity :

This assay has high sensitivity and excellent specificity for detection of Attractin (ATRN). No significant cross-reactivity or interference between Attractin (ATRN) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.147ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Attractin (ATRN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Attractin (ATRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Attractin (ATRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Attractin (ATRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Attractin (ATRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Attractin (ATRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

75516722

ncbi acc num :

AAI01706.1

uniprot acc num :

O75882

ncbi mol weight :

133,702 Da

ncbi summary :

Multiple transcript variants encoding different isoforms exist for this gene. One of the isoforms is a membrane-bound protein with sequence similarity to the mouse mahogany protein, a receptor involved in controlling obesity. The other two isoforms are secreted proteins involved in the initial immune cell clustering during inflammatory responses that may regulate the chemotactic activity of chemokines. [provided by RefSeq, May 2011]

uniprot summary :

ATRN: Involved in the initial immune cell clustering during inflammatory response and may regulate chemotactic activity of chemokines. May play a role in melanocortin signaling pathways that regulate energy homeostasis and hair color. Low-affinity receptor for agouti. Has a critical role in normal myelination in the central nervous system. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: extracellular space; integral to plasma membrane; cytoplasm; plasma membrane. Molecular Function: receptor activity; carbohydrate binding. Biological Process: myelination; pigmentation; cerebellum development; regulation of multicellular organism growth; response to oxidative stress; inflammatory response

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage