catalog number :
MBS2022691
products type :
ELISA Kit
products full name :
Visfatin (VF) ELISA Kit
products short name :
[Visfatin (VF)]
products name syn :
[Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase]
other names :
[visfatin; Visfatin; Visfatin]
products gene name :
[VF]
uniprot entry name :
S5MI21_LARCR
specificity :
This assay has high sensitivity and excellent specificity for detection of Visfatin (VF). No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Standard Curve/Testing Data
other info1 :
Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma and Other Biological Fluids. Detection Range: 0.156-10ng/mL. Sensitivity: < 0.059ng/mL
other info2 :
Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visfatin (VF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visfatin (VF) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%
products categories :
Metabolic pathway; Cardiovascular biology; Hepatology
products description :
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visfatin (VF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visfatin (VF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visfatin (VF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Visfatin (VF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AGR51149.1
ncbi mol weight :
55,759 Da
size5 :
10x96-Strip-Wells