ELISA/assay

Haptoglobin (Hpt) ELISA Kit

MBS2022684

24-Strip-Wells

210 USD

ELISA Kit

Haptoglobin (Hpt) ELISA Kit

[Haptoglobin (Hpt)]

[HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin]

[haptoglobin; Haptoglobin; haptoglobin; haptoglobin; Zonulin]

[Hpt]

[HP; HP; BP; HPA1S; HP2ALPHA2]

HPT_HUMAN

Human

347

This assay has high sensitivity and excellent specificity for detection of H4. No significant cross-reactivity or interference between H4 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Canine Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.055ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level H4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level H4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Infection immunity; Hematology

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of H4 in canine tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to H4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to H4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of H4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

386783

AAA88080.1

P00738

38,452 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600); Amb2 Integrin Signaling Pathway (137945)

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16q22.2. Cellular Component: extracellular space; extracellular region. Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity. Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response. Disease: Anhaptoglobinemia

24-Strip-Wells

210 USD

48-Strip-Wells

340

96-Strip-Wells

450

5x96-Strip-Wells

1775

10x96-Strip-Wells

3200