Interleukin 17 (IL17) ELISA Kit | MyBioSource MBS2022678 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Interleukin 17 (IL17) ELISA Kit

catalog :

MBS2022678

quantity :

24-Strip-Wells

price :

265 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2022678

products type :

ELISA Kit

products full name :

Interleukin 17 (IL17) ELISA Kit

products short name :

[Interleukin 17 (IL17)]

products name syn :

[IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8]

other names :

[interleukin-17, partial; Interleukin-17; interleukin-17A; interleukin 17A; Interleukin-17]

products gene name :

[IL17]

other gene names :

[IL17A; IL17; IL17; CTLA8; IL-17; IL-17A]

uniprot entry name :

Q5QEX9_HUMAN

reactivity :

Rat

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17). No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 15.6-1000pg/mL. Sensitivity: < 6.3pg/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Cytokine; Infection immunity

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 17 (IL17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 17 (IL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 17 (IL17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Interleukin 17 (IL17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

55139064

ncbi acc num :

AAV41220.1

ncbi mol weight :

933 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL17 Signaling Pathway (920968); IL23-mediated Signaling Events Pathway (138000); IL27-mediated Signaling Events Pathway (137929); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269); TCR Signaling Pathway (198862)

ncbi summary :

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

uniprot summary :

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p12. Cellular Component: extracellular space; external side of plasma membrane. Molecular Function: cytokine activity. Biological Process: cell death; cell-cell signaling; apoptosis; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; protein amino acid glycosylation; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response

size1 :

24-Strip-Wells

price1 :

265 USD

size2 :

48-Strip-Wells

price2 :

490

size3 :

96-Strip-Wells

price3 :

655

size4 :

5x96-Strip-Wells

price4 :

2660

size5 :

10x96-Strip-Wells

price5 :

4795

more info or order :

MyBioSource product webpage