Major Basic Protein (MBP) ELISA Kit | MyBioSource MBS2022666 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Major Basic Protein (MBP) ELISA Kit

catalog :

MBS2022666

quantity :

24-Strip-Wells

price :

265 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2022666

products type :

ELISA Kit

products full name :

Major Basic Protein (MBP) ELISA Kit

products short name :

[Major Basic Protein (MBP)]

products name syn :

[PRG2; BMPG; Proteoglycan 2; Bone Marrow,Natural Killer Cell Activator; Eosinophil Granule Major Basic Protein; Proteoglycan 2,Bone Marrow; Pregnancy-associated major basic]

other names :

[major basic protein; Bone marrow proteoglycan; bone marrow proteoglycan; proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein); Proteoglycan 2]

products gene name :

[MBP]

other gene names :

[PRG2; PRG2; MBP; BMPG; MBP1; MBP; BMPG; EMBP; MBP]

uniprot entry name :

PRG2_HUMAN

reactivity :

Mouse

sequence length :

211

specificity :

This assay has high sensitivity and excellent specificity for detection of MBP. No significant cross-reactivity or interference between MBP and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: <0.249ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MBP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MBP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Infection immunity; Rheumatology; Dermatology

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MBP in mouse serum, plasma, tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MBP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

187415

ncbi acc num :

AAA36203.1

uniprot acc num :

P13727

ncbi mol weight :

23,901 Da

ncbi pathways :

Asthma Pathway (83120); Asthma Pathway (532)

ncbi summary :

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. The encoded protein contains a peptide that displays potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

uniprot summary :

PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. Protein type: Secreted, signal peptide; Secreted; Vesicle. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: transport vesicle; extracellular region. Molecular Function: heparin binding; carbohydrate binding. Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; defense response to bacterium; immune response; defense response to nematode

size1 :

24-Strip-Wells

price1 :

265 USD

size2 :

48-Strip-Wells

price2 :

490

size3 :

96-Strip-Wells

price3 :

655

size4 :

5x96-Strip-Wells

price4 :

2660

size5 :

10x96-Strip-Wells

price5 :

4795

more info or order :

MyBioSource product webpage