ELISA/assay

Matrix Metalloproteinase 14 (MMP14) ELISA Kit

MBS2022640

48-Strip-Wells

565 USD

ELISA Kit

Matrix Metalloproteinase 14 (MMP14) ELISA Kit

Matrix Metalloproteinase 14 (MMP14)

MMP-X1; MT1-MMP; MTMMP1; Membrane Inserted; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase

matrix metalloproteinase-14 preproprotein; Matrix metalloproteinase-14; matrix metalloproteinase-14; matrix metallopeptidase 14 (membrane-inserted); MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP

MMP14

MMP14; MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP

MMP14_HUMAN

Human

582

This assay has high sensitivity and excellent specificity for detection of MMP14. No significant cross-reactivity or interference between MMP14 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. The standard curve concentrations used for the ELISA's were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.12ng/mL, 1.56ng/mL. Sensitivity: 0.57ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP14 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP14 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP14 in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MMP14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MMP14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4826834

NP_004986.1

NM_004995.3

P50281

65,894 Da

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); GnRH Signaling Pathway (83091); GnRH Signaling Pathway (502); HIF-2-alpha Transcription Factor Network Pathway (137956); Matrix Metalloproteinases Pathway (198900); Senescence And Autophagy Pathway (198780)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.4.24.80; Protease. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular matrix; focal adhesion; integral to plasma membrane; Golgi lumen; cytoplasm; melanosome; plasma membrane. Molecular Function: integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity. Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; male gonad development; negative regulation of focal adhesion formation; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; collagen catabolic process; extracellular matrix disassembly; ovarian follicle development; regulation of transcription, DNA-dependent; branching morphogenesis of a tube; response to estrogen stimulus; response to mechanical stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; response to oxidative stress; angiogenesis; astrocyte cell migration; positive regulation of cell migration; lung development; endochondral ossification. Disease: Winchester Syndrome

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340