catalog number :
MBS2022629
products type :
ELISA Kit
products full name :
Heat Shock Protein 90 (HSP90) ELISA Kit
products short name :
[Heat Shock Protein 90 (HSP90)]
products name syn :
[HtpG; Hsp-90 NTD; N-Terminal Domain]
other names :
[heat shock protein 90; Chaperone protein HtpG; Heat shock protein HtpG]
products gene name :
[HSP90]
other gene names :
[htpG]
uniprot entry name :
I2AXA7_FRANT
specificity :
This assay has high sensitivity and excellent specificity for detection of HSP70. No significant cross-reactivity or interference between HSP70 and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.12-200ng/mL. Sensitivity: 1.33ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HSP70 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HSP70 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products categories :
Signal transduction; Infection immunity; Hematology; Developmental science
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HSP70 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to HSP70. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HSP70. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP70, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HSP70 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AFJ42804.1
ncbi mol weight :
72,226 Da
uniprot summary :
Molecular chaperone. Has ATPase activity.
size5 :
10x96-Strip-Wells