Semaphorin 3F (SEMA3F) ELISA Kit | MyBioSource MBS2022622 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Semaphorin 3F (SEMA3F) ELISA Kit

catalog :

MBS2022622

quantity :

48-Strip-Wells

price :

610 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022622

products type :

ELISA Kit

products full name :

Semaphorin 3F (SEMA3F) ELISA Kit

products short name :

Semaphorin 3F (SEMA3F)

products name syn :

SEMA-IV; SEMA4; SEMAK; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3F

other names :

semaphorin-3F; Semaphorin-3F; semaphorin-3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; Sema III/F; Semaphorin IV; Sema IV

products gene name :

SEMA3F

other gene names :

SEMA3F; SEMA3F; SEMA4; SEMAK; SEMA-IV; Sema IV

uniprot entry name :

SEM3F_HUMAN

reactivity :

Human

sequence length :

754

specificity :

This assay has high sensitivity and excellent specificity for detection of SEMA3F. No significant cross-reactivity or interference between SEMA3F and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.6-1,000pg/mL. The standard curve concentrations used for the ELISA's were 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL. Sensitivity: 6.7pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA3F were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA3F were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA3F in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEMA3F. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SEMA3F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA3F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SEMA3F in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

188497703

ncbi acc num :

NP_004177.3

ncbi gb acc num :

NM_004186.3

uniprot acc num :

Q13275

ncbi mol weight :

85,064 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476)

ncbi summary :

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM, Mar 2008]

uniprot summary :

SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: extracellular space; membrane. Molecular Function: chemorepellent activity; receptor activity. Biological Process: axon extension involved in axon guidance; axon guidance; facial nerve structural organization; trigeminal nerve structural organization; branchiomotor neuron axon guidance; negative chemotaxis; nerve development; negative regulation of axon extension involved in axon guidance

size1 :

48-Strip-Wells

price1 :

610 USD

size2 :

96-Strip-Wells

price2 :

830

size3 :

5x96-Strip-Wells

price3 :

3265

size4 :

10x96-Strip-Wells

price4 :

5870

more info or order :

MyBioSource product webpage