ELISA/assay

Adrenomedullin (ADM) ELISA Kit

MBS2022600

24-Strip-Wells

255 USD

ELISA Kit

Adrenomedullin (ADM) ELISA Kit

[Adrenomedullin (ADM)]

[AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide]

[adrenomedullin; ADM; ADM; adrenomedullin; Adrenomedullin; AM]

[ADM]

[ADM; ADM; AM; PAMP; AM; AM; PAMP; ProAM-N20]

ADML_HUMAN

Human

185

This assay has high sensitivity and excellent specificity for detection of adrenomedullin. No significant cross-reactivity or interference between adrenomedullin and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Quantitative Competitive. Detection Range: 12.35-1,000pg/mL. Sensitivity: 4.91pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level adrenomedullin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level adrenomedullin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Endocrinology; Hormone metabolism

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of adrenomedullin in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to adrenomedullin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled adrenomedullin and unlabeled adrenomedullin (Standards or samples) with the pre-coated antibody specific to adrenomedullin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of adrenomedullin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of adrenomedullin in the sample.

765330

AAC60642.1

P35318

20,420 Da

Calcitonin-like Ligand Receptors Pathway (106379); Class B/2 (Secretin Family Receptors) Pathway (106378); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Myometrial Relaxation And Contraction Pathways (198759)

The protein encoded by this gene is a preprohormone which is cleaved to form two biologically active peptides, adrenomedullin and proadrenomedullin N-terminal 20 peptide. Adrenomedullin is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. [provided by RefSeq, Aug 2014]

ADM: AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. Belongs to the adrenomedullin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.4. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: protein binding; adrenomedullin receptor binding; hormone activity; receptor binding. Biological Process: developmental growth; blood circulation; positive regulation of apoptosis; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; female pregnancy; signal transduction; positive regulation of vasodilation; response to insulin stimulus; negative regulation of cell proliferation; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; cell-cell signaling; receptor internalization; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; response to wounding; vasculogenesis; regulation of the force of heart contraction; aging; hormone secretion; organ regeneration; progesterone biosynthetic process; androgen metabolic process; neurite regeneration; positive regulation of heart rate; negative regulation of vasoconstriction; odontogenesis of dentine-containing teeth; response to starvation; positive regulation of angiogenesis; G-protein coupled receptor internalization; neural tube closure; negative regulation of vascular permeability; response to hypoxia; cAMP biosynthetic process; response to cold

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445