ELISA/assay

Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) ELISA Kit

MBS2022553

96 Strip Wells

780 USD

ELISA Kit

Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) ELISA Kit

Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7)

P2X7; P2X Purinoceptor 7; ATP receptor; Purinergic receptor

P2X purinoceptor 7; P2X purinoceptor 7; P2X purinoceptor 7; purinergic receptor P2X, ligand gated ion channel, 7; ATP receptor; P2Z receptor; Purinergic receptor

P2RX7

P2RX7; P2RX7; P2X7; P2X7

P2RX7_HUMAN

Human

505

This assay has high sensitivity and excellent specificity for detection of Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). No significant cross-reactivity or interference between Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 0.059ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

300068987

NP_002553.3

NM_002562.5

Q99572

58,239 Da

Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Immune System Pathway 106386!!Inflammasomes Pathway 366166!!Innate Immune System Pathway 106387!!Neuroactive Ligand-receptor Interaction Pathway 83053!!Neuroactive Ligand-receptor Interaction Pathway 462!!Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways 366164!!The NLRP3 Inflammasome Pathway 366167

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

96 Strip Wells

780 USD