ELISA/assay

Prokineticin 2 (PK2) ELISA Kit

MBS2022546

24-Strip-Wells

255 USD

ELISA Kit

Prokineticin 2 (PK2) ELISA Kit

[Prokineticin 2 (PK2)]

[PK-2; Bv8; MIT1; KAL4; Protein Bv8 Homolog]

[prokineticin-2 isoform a; Prokineticin-2; prokineticin-2; prokineticin 2; Protein Bv8 homolog]

[PK2]

[PROK2; PROK2; BV8; HH4; PK2; KAL4; MIT1; BV8; PK2]

PROK2_HUMAN

Human

108

This assay has high sensitivity and excellent specificity for detection of PIP3. No significant cross-reactivity or interference between PIP3 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 6.17-500ng/mL. Sensitivity: 2.94ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PIP3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PIP3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Neuroscience

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of PIP3 in serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PIP3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PIP3 and unlabeled PIP3 (Standards or samples) with the pre-coated antibody specific to PIP3. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PIP3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PIP3 in the sample.

187167261

NP_001119600.1

NM_001126128.1

Q9HC23

11,659 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114)

This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PROK2: May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle. Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4); also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin- releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis. Belongs to the AVIT (prokineticin) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3p13. Cellular Component: extracellular region. Molecular Function: G-protein-coupled receptor binding. Biological Process: circadian rhythm; activation of MAPK activity; sensory perception of pain; chemotaxis; G-protein coupled receptor protein signaling pathway; cell proliferation; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spermatogenesis; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; negative regulation of apoptosis. Disease: Hypogonadotropic Hypogonadism 4 With Or Without Anosmia

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445