ELISA/assay

Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2022516

24-Strip-Wells

245 USD

ELISA Kit

Connective Tissue Growth Factor (CTGF) ELISA Kit

[Connective Tissue Growth Factor (CTGF)]

[CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2]

[connective tissue growth factor; Connective tissue growth factor; connective tissue growth factor; connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Insulin-like growth factor-binding protein 8; IBP-8; IGF-binding protein 8; IGFBP-8]

[CTGF]

[CTGF; CTGF; CCN2; NOV2; HCS24; IGFBP8; CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8]

CTGF_HUMAN

Human

322

This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF). No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids. Detection Range: 7.8-500pg/mL. Sensitivity: < 3.5pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Cytokine

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connective Tissue Growth Factor (CTGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Connective Tissue Growth Factor (CTGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

180924

AAA91279.1

P29279

35,205 Da

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544); TGF-beta Receptor Signaling Pathway (138024)

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Cell adhesion. Chromosomal Location of Human Ortholog: 6q23.1. Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; cis-Golgi network; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; extracellular region; cell cortex; cytosol. Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; protein binding; growth factor activity; fibronectin binding. Biological Process: response to peptide hormone stimulus; epidermis development; response to anoxia; cell-matrix adhesion; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of caspase activity; cellular lipid metabolic process; response to estradiol stimulus; cell-cell signaling; positive regulation of stress fiber formation; positive regulation of cell proliferation; tissue homeostasis; response to wounding; response to glucose stimulus; angiogenesis; regulation of cell growth; cell differentiation; cell adhesion; cartilage condensation; positive regulation of cell activation; integrin-mediated signaling pathway; transcription initiation from RNA polymerase II promoter; cell migration; ossification; fibroblast growth factor receptor signaling pathway; organ senescence; response to mineralocorticoid stimulus; response to amino acid stimulus; gene expression; positive regulation of protein amino acid phosphorylation; regulation of chondrocyte differentiation; positive regulation of cell differentiation; lung development

24-Strip-Wells

245 USD

48-Strip-Wells

415

96-Strip-Wells

555

5x96-Strip-Wells

2220

10x96-Strip-Wells

4000